Complete Genome Sequence of Carbonic Anhydrase Producing Psychrobacter sp. SHUES1

Recent advances in biotechnology have allowed the study of new bacterial strains, which can produce enzymes that can be used in the bioremediation of heavy metals. Microbially induced carbonate precipitation (MICP) is a recent well-recognized process that has the potential to precipitate heavy metals, mainly those with a valency of +2 (Kumari et al., 2016). There are two enzymes, urease, and carbonic anhydrase, that play an important role in the MICP process. The role of carbonic anydrase (EC 4.2.1.1) in MICP is generally underestimated and most of the studies in past mainly focus on urease-producing microorganisms (Li et al., 2013, 2014; Kumari et al., 2014). In the present study, Psychrobacter sp. SHUES1 was isolated from frozen alkaline soil sample collected at Shanghai, China. This bacterium produced lipase and protease at 4 • C in a plate assay. The ability of Psychrobacter sp. to show extracellular lipolytic activity at low temperatures is widely known (Xuezheng et al., 2010); however, the remarkable property of this strain was in the precipitation of heavy metals including cadmium and zinc in parallel to the MICP process. Therefore, to know the type of enzyme or genes involved in the process of metal precipitation, this research aims to sequence the whole genome of Psychrobacter sp. SHUES1, and thus provide a genomic insight into its behavior. Genomic DNA from Psychrobacter sp. SHUES1 was extracted using the DNeasy Blood & Tissue Kit (Qiagen, USA), and its quantity and quality were evaluated on the Qubit. The extracted DNA was subjected to whole-genome shotgun sequencing using the NEBNext Ultra DNA Library Prep Kit (Illumina, San Diego, CA). Library construction was performed with the following process: DNA fragmentation, end repair, adding " A " to the 3 ′ end, adaptor ligation and amplification. After library construction, the generated cluster was sequenced on an Illumina HiSeq2500 sequencing system, according to a paired end 2 × 125 nt multiplex program. 13,716,515 raw reads resulted in 13,144,818 quality-filtered trimmed reads, yielding a not less than 3 Mb genome size. De novo genome assembly was performed using SPAdes-3.5.0. After purification, the assembly produced 3,115,590 bp of sequence across 115 contigs with an N50 of 47,049 bp, with a longest sequence of 182,144 bp, and a G+C content of 43.5% (Table 1). Gene prediction and annotation were carried out using Prodigal_v2.6.1, blastp in the National Center for Biotechnology Information (NCBI) " nr " database. Gene ontology (GO) …